Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10755639 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
The type II Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to play a vital role in cellular regulation in mammalian cells. Two genes CMK1 and CMK2 in the Candida albicans genome encode homologues of mammalian CaMKs. In this work, we constructed the cmk1Î/Î, the cmk2Î/Î and the cmk1Î/Îcmk2Î/Î mutants and found that CaMKs function in cell wall integrity (CWI) and cellular redox regulation. Loss of either CMK1 or CMK2, or both resulted in increased expression of CWI-related genes under Calcofluor white (CFW) treatment. Besides, CaMKs are essential for the maintenance of cellular redox balance. Disruption of either CMK1 or CMK2, or both not only led to a significant increase of intracellular ROS levels, but also led to a decrease of the mitochondrial membrane potential (MMP), suggesting the important roles that CaMKs play in the maintenance of the mitochondrial function.
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Biochemistry
Authors
Xiaohui Ding, Qilin Yu, Bing Zhang, Ning Xu, Chang Jia, Yijie Dong, Yulu Chen, Laijun Xing, Mingchun Li,