Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10756683 | Biochemical and Biophysical Research Communications | 2013 | 22 Pages |
Abstract
Endoplasmic reticulum (ER) stress is associated with the development of diabetes. The present study sought to investigate the effect of Liraglutide, a glucagon like peptide 1 analogue, on ER stress in β-cells. We found that Liraglutide protected the pancreatic INS-1 cells from thapsigargin-induced ER stress and the ER stress associated cell apoptosis, mainly by suppressing the PERK and IRE1 pathways. We further tested the effects of Liraglutide in the Akita mouse, an ER-stress induced type 1 diabetes model. After administration of Liraglutide for 8 weeks, p-eIF2α and p-JNK were significantly decreased in the pancreas of the Akita mouse, while the treatment showed no significant impact on the levels of insulin of INS-cells. Taken together, our findings suggest that Liraglutide may protect pancreatic cells from ER stress and its related cell death.
Keywords
double-stranded RNA-activated protein kinase (PKR)-like ER kinaseATF-6Akita mouseGLP-1ThapsigarginUPRIRE1inositol requiring enzyme 1eIF2α3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideBiPC/EBP homologous proteinMTTER stressDiabetesCHOPINS-1 cellsendoplasmic reticulumeukaryotic translation initiation factor 2αactivating transcription factor 6LiraglutideUnfolded protein responsePERKglucagon-like peptide 1
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Authors
Linlin Zhao, Hao Guo, Hong Chen, Robert B. Petersen, Ling Zheng, Anlin Peng, Kun Huang,