Generation of robust vascular networks from cardiovascular blast populations derived from human induced pluripotent stem cells in vivo and ex vivo organ culture system

By using cell-sheet technology, we observed that CBP with CD31+ cells to CD90+ cells in the ratio of 1:1.5 could reproducibly form robust vascular networks in vivo and ex vivo organ culture system. To our knowledge, this is the first report demonstrating the generation of vascular network from hiPSCs in ex vivo organ culture system that correlates closely with in vivo results. Our results suggest that CBP provides a promising approach for studying vasculogenesis and subsequently can be used in regenerative medicine.