Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10756750 | Biochemical and Biophysical Research Communications | 2013 | 4 Pages |
Abstract
All influenza viral neuraminidases (NA) of both type A and B viruses have only one universally conserved sequence located between amino acids 222-230. A monoclonal antibody against this region has been previously reported to provide broad inhibition against all nine subtypes of influenza A NA; yet its inhibitory effect against influenza B viral NA remained unknown. Here, we report that the monoclonal antibody provides a broad inhibition against various strains of influenza B viruses of both Victoria and Yamagata genetic lineage. Moreover, the growth and NA enzymatic activity of two drug resistant influenza B strains (E117D and D197E) are also inhibited by the antibody even though these two mutations are conformationally proximal to the universal epitope. Collectively, these data suggest that this unique, highly-conserved linear sequence in viral NA is exposed sufficiently to allow access by inhibitory antibody during the course of infection; it could represent a potential target for antiviral agents and vaccine-induced immune responses against diverse strains of type B influenza virus.
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Authors
Tracey M. Doyle, Changgui Li, Doris J. Bucher, Anwar M. Hashem, Gary Van Domselaar, Junzhi Wang, Aaron Farnsworth, Yi-Min She, Terry Cyr, Runtao He, Earl G. Brown, Aeron C. Hurt, Xuguang Li,