Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10756910 | Biochemical and Biophysical Research Communications | 2014 | 4 Pages |
Abstract
The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations.
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Authors
Wataru Fujii, Asuka Onuma, Koji Sugiura, Kunihiko Naito,