Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10756986 | Biochemical and Biophysical Research Communications | 2013 | 6 Pages |
Abstract
Furthermore, we examined the effects of anti-malarial drugs in combination with an antimitotic drug, vinblastine (VIN) on the sensitisation of resistant KBV20C cells. Using viability assay, microscopic observation, assessment of cleaved PARP, and Hoechst staining, we identified that two anti-malarial drugs, PRI and MEF, highly sensitized KBV20C-resistant cells to VIN treatment. Moreover, PRI- or MEF-induced sensitisation was not observed in VIN-treated sensitive KB parent cells, suggesting that the observed effect is specific to resistant cancer cells. We demonstrated that the PRI and MEF sensitisation mechanism mainly depends on the inhibition of p-glycoprotein (P-gp). Our findings may contribute to the development of anti-malarial drug-based combination therapies for patients resistant to anti-mitotic drugs.
Keywords
AtovaquoneFACSP-gpFBSPRLPBSATOMEFTCAChlDMSOP-glycoproteinSDS–PAGEArtesunatetrichloroacetic acidsodium dodecyl sulfate–polyacrylamide gel electrophoresisDOYdoxycyclinefluorescence-activated cell sortingRoom temperatureDimethylsulfoxidefetal bovine serumVINPhosphate buffered salineVerDrug-resistancemefloquineARTVerapamilVinblastinePrimaquineChloroquine
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Authors
Ju-Hwa Kim, Ae-Ran Choi, Yong Kee Kim, Sungpil Yoon,