Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10757006 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
MicroRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. In this study, we established the miRNAs-expression patterns of primary hepatocytes in vitro under stimulation of epidermal growth factor (EGF), and found that microRNA-21 (miR-21) was appreciably up-regulated and peaked at 12Â h. In addition, we further presented evidences indicating that miR-21 promotes primary hepatocyte proliferation through in vitro transfecting with miR-21 mimics or inhibitor. We further demonstrated that phosphatidylinositol 3â²-OH kinase (PI3K)/Akt signaling was altered accordingly, it is, by targeting phosphatase and tensin homologue deleted on chromosome 10, PI3K/Akt signaling is activated by miR-21 to accelerate hepatocyte rapid S-phase entry and proliferation in vitro.
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Authors
Bai Yan-nan, Yu Zhao-yan, Luo Li-xi, Yi jiang, Xia Qing-jie, Zeng Yong,