Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10757040 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
- We detailedly examine temperature effects of Fluo-3 AM labelling in adherent cells.
- 4 °C Loading and 20 °C de-esterification of Fluo-3 AM yields brighter nuclei.
- Brighter nuclei labelling by Fluo-3 AM also depends on cell adhesion quality.
- A qualitative model of the brighter nucleus is proposed.
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Authors
Guixian Meng, Leiting Pan, Cunbo Li, Fen Hu, Xuechen Shi, Imshik Lee, Irena Drevenšek-Olenik, Xinzheng Zhang, Jingjun Xu,