Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10757360 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
KS483 cells were cultured under osteogenic conditions in the presence of compounds that either stimulate or inhibit osteoblast differentiation and thereby matrix mineralization. After 21Â days of differentiation, fluorescence of stained cultures was quantified with a near-infrared imager and compared to Alizarin Red S quantification. Fluorescence of both probes closely correlated to Alizarin Red S staining in both inhibiting and stimulating conditions. In addition, both compounds displayed specificity for mineralized nodules. We therefore conclude that this method of quantification of bone mineralization using fluorescent compounds is a good alternative for the Alizarin Red S staining.
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Authors
Martiene J.C. Moester, Monique A.E. Schoeman, Ineke B. Oudshoorn, Mara M. van Beusekom, Isabel M. Mol, Eric L. Kaijzel, Clemens W.G.M. Löwik, Karien E. de Rooij,