Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10757804 | Biochemical and Biophysical Research Communications | 2013 | 20 Pages |
Abstract
Prelamin A accumulation causes nuclear abnormalities, impairs nuclear functions, and eventually promotes cellular senescence. However, the underlying mechanism of how prelamin A promotes cellular senescence is still poorly understood. Here we carried out a yeast two-hybrid screen using a human skeletal muscle cDNA library to search for prelamin A binding partners, and identified FAM96B as a prelamin A binding partner. The interaction of FAM96B with prelamin A was confirmed by GST pull-down and co-immunoprecipitation experiments. Furthermore, co-localization experiments by fluorescent confocal microscopy revealed that FAM96B colocalized with prelamin A in HEK-293 cells. Taken together, our data demonstrated the physical interaction between FAM96B and prelamin A, which may provide some clues to the mechanisms of prelamin A in premature aging.
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Authors
Xing-Dong Xiong, Junwen Wang, Huiling Zheng, Xia Jing, Zhenjie Liu, Zhongjun Zhou, Xinguang Liu,