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Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR

Article ID Journal Published Year Pages File Type
10758279 Biochemical and Biophysical Research Communications 2013 5 Pages PDF
Abstract

- We developed enChIP for purification of specific genomic regions.
- enChIP using the CRISPR system efficiently isolates specific genomic regions.
- enChIP-mass spectrometry (enChIP-MS) identified proteins associated with those loci.
Keywords
CRISPRgRNAdCas9Mass spectrometry
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR
Authors
Toshitsugu Fujita, Hodaka Fujii,
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Journal
Biochemical and Biophysical Research Communications
Journal: Biochemical and Biophysical Research Communications
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