Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10758362 | Biochemical and Biophysical Research Communications | 2013 | 4 Pages |
Abstract
Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50 °C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97 Ã
) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP+ reductase or cytochrome c.
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Authors
Yuko Ueno, Takashi Matsumoto, Akihito Yamano, Takeo Imai, Yukio Morimoto,