Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10758664 | Biochemical and Biophysical Research Communications | 2013 | 6 Pages |
Abstract
Phosphatidylinositol 4 phosphate 5 kinase 1α (PIP5K) is mainly localized in the cytosol and plasma membrane. Studies have also indicated its prominent association with nuclear speckles. The exact nature of this nuclear pool of PIP5K is not clear. Using biochemical and microscopic techniques, we have demonstrated that the nuclear pool of PIP5K is modified by SUMO-1 in HEK-293 cells stably expressing PIP5K. Moreover, this SUMOylated pool of PIP5K increased during apoptosis. PolySUMO-2 chain conjugated PIP5K was detected by pull-down experiment using affinity-tagged RNF4, a polySUMO-2 binding protein, during late apoptosis.
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Authors
Rajarshi Chakrabarti, Debajit Bhowmick, Varsha Bhargava, Kaushik Bhar, Anirban Siddhanta,