Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10758965 | Biochemical and Biophysical Research Communications | 2013 | 6 Pages |
Abstract
The cellular response to 100Â ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in intracellular Ca2+, an increase in reactive oxygen species (ROS), DNA fragmentation and caspase activation. 100Â ns, 30Â kV/cm nsPEF stimulates an increase in ROS proportional to the pulse number. This increase is inhibited by the anti-oxidant, Trolox, as well as the presence of Ca2+ chelators in the intracellular and extracellular media. This suggests that the nsPEF-triggered Ca2+ increase is required for ROS generation.
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Authors
Richard Nuccitelli, Kaying Lui, Mark Kreis, Brian Athos, Pamela Nuccitelli,