Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10759462 | Biochemical and Biophysical Research Communications | 2013 | 6 Pages |
Abstract
The underlying mechanisms of microRNA deregulation in cancer cells include epigenetic modifications, which play a crucial role in carcinogenesis. We demonstrate that numerous microRNAs are induced in renal cell carcinoma cell lines after treatment with inhibitors of the DNA-methyltransferase (5-aza-2â²-deoxycytidine) and the histone-deacetylase (suberoylanilide hydroxamic acid). We provide evidence that enrichment of H3 and H3K18 acetylation at the miR-9 promoter is causative for re-expression, while DNA hypermethylation remains unchanged. Our experiments show that the treatment with the epigenetic drugs causes re-expression of silenced microRNAs with putative tumor suppressive function in ccRCC cell lines.
Keywords
TBS-tweenccRCCSAHAHDACMIRDnmtTBST5-Aza-2′-deoxycytidineDNA methyltransferaseSDS–PAGEHistone acetylationSuberoylanilide hydroxamic acidsodium dodecyl sulphate–polyacrylamide gel electrophoresischromatin immunoprecipitationRINClear cell renal cell carcinomaDNA methylationMicroRNAhistone deacetylaseCHiPRenal cell carcinoma
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Authors
Miriam Schiffgen, Doris H. Schmidt, Alexander von Rücker, Stefan C. Müller, Jörg Ellinger,