Presence of a novel exon 2E encoding a putative transmembrane protein in human IL-33 gene

Article ID	Journal	Published Year	Pages	File Type
10759877	Biochemical and Biophysical Research Communications	2013	6 Pages	PDF

Abstract

âº Promoter usage of IL-33 is dependent on cell type. âº A novel exon 2E is found in the conventional intron 2 of IL-33 gene. âº Exon 2E encodes putative transmembrane protein of 131 amino acids. âº Expression of exon 2E is suggested to be dependent on differentiation of HUVEC cells.

Keywords

DMEM Nonidet P-40 NP-40 ST2 IL-33 RT-PCR GAPDH FBS cDNA Complementary DNA SDS–PAGE EDTA Ethylenediaminetetraacetic acid sodium dodecyl sulfate–polyacrylamide gel electrophoresis Differentiation fetal bovine serum Dulbecco’s modified eagle’s medium reverse transcription-polymerase chain reaction Transmembrane protein Splicing glyceraldehyde-3-phosphate dehydrogenase