Article ID Journal Published Year Pages File Type
10759894 Biochemical and Biophysical Research Communications 2013 5 Pages PDF
Abstract
► DHP A is a dual function protein capable of oxidizing toxic trihalophenols to dihaloquinones. ► Kinetics are controlled by electrostatics since enzyme and substrate are both negatively charged. ► Stark probe molecule, 4-mercaptobenzonitrile, used to probe protein surface electrostatics. ► MD simulations confirm that Stark probe reports changes to overall protein electrostatics. ► Linear correlation between vibrational Stark effect and kinetic data reveal electrostatic control in DHP.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
Catalytic efficiency of dehaloperoxidase A is controlled by electrostatics - application of the vibrational Stark effect to understand enzyme kinetics
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