Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10760128 | Biochemical and Biophysical Research Communications | 2013 | 5 Pages |
Abstract
⺠The orthogonal synthetase derived from M. jannaschii is an inefficient catalyst. ⺠The estimated turnover number for the orthogonal synthetase is 5.4 Ã 10â5 sâ1. ⺠The o-tRNA concentration in the standard cell-free platform is 0.6 ± 0.1 μM. ⺠In vivo- and in vitro-produced orthogonal tRNA seem equally competent.
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Authors
Cem Albayrak, James R. Swartz,