Article ID Journal Published Year Pages File Type
10760128 Biochemical and Biophysical Research Communications 2013 5 Pages PDF
Abstract
► The orthogonal synthetase derived from M. jannaschii is an inefficient catalyst. ► The estimated turnover number for the orthogonal synthetase is 5.4 × 10−5 s−1. ► The o-tRNA concentration in the standard cell-free platform is 0.6 ± 0.1 μM. ► In vivo- and in vitro-produced orthogonal tRNA seem equally competent.
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