Visualization of cyclooxygenase-2 using a 2,3-diarylsubstituted indole-based inhibitor and confocal laser induced cryofluorescence microscopy at 20Â K in melanoma cells in vitro

► Application of both cryofluorescence microscopy and spectroscopy in melanoma cells. ► Development of a sample chamber providing constant cryogenic temperatures (±1 K). ► Use of fluorescent 2,3-diarylsubstituted indole cyclooxygenase-2 inhibitors. ► Sensitive visualization of cyclooxygenase-2 expression at 20 K.