| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10760590 | Biochemical and Biophysical Research Communications | 2012 | 5 Pages |
Abstract
⺠First experimental determination of Förster distance (R0) for enhanced BRET systems. ⺠Effect of brighter BRET components RLuc2, RLuc8 and Venus was assessed. ⺠Using brighter BRET components substantially increased (25%) R0 of the BRET1 system. ⺠Using brighter BRET components marginally increased (2-9%) R0 of the BRET2 system. ⺠Brighter BRET components improve the different weaknesses of BRET1 and BRET2 systems.
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Authors
Helen Dacres, Michelle Michie, Jian Wang, Kevin D.G. Pfleger, Stephen C. Trowell,