Engagement of T-cell antigen receptor and CD4/CD8 co-receptors induces prolonged STAT activation through autocrine/paracrine stimulation in human primary T cells

Article ID	Journal	Published Year	Pages	File Type
10760637	Biochemical and Biophysical Research Communications	2012	5 Pages	PDF

Abstract

âº CD3/CD4/CD8 engagement induces sequential STAT1/3/5 activation in human primary T cells. âº Delayed STAT activation is due to autocrine/paracrine stimulation. âº IFN-Î³ contributes to STAT1 activation in TCR-stimulated cells. âº Prolonged STAT activation correlates with the absence of CIS and SOCS3 proteins. âº Exogenous SOCS3, but not CIS, inhibits TCR signaling.

Keywords

NFAT EMSA sis-inducible element Cytokine-inducible SH2-containing protein LCK ZAP-70 Nuclear factor of activated T cell SOCS MGE PBS CIS TCr ECL Janus kinase SiE Electrophoretic mobility shift assay interferon IFN interleukin enhanced chemiluminescence suppressor of cytokine signaling Cytokine Signal transducer and activator of transcription major histocompatibility complex MHC Phosphate-buffered saline JAK T-cell antigen receptor