A new method for establishing stable cell lines and its use for large-scale production of human guanylyl cyclase-B receptor and of the extracellular domain

Article ID	Journal	Published Year	Pages	File Type
10760643	Biochemical and Biophysical Research Communications	2012	6 Pages	PDF

Abstract

âº Stable cell lines highly expressing GC-B and the ECD were established by using FACS. âº The ECD was purified by affinity chromatography using modified CNPs. âº The full-length GC-B in membranes and the purified ECD had expected activities.

Keywords

FACS ANP ECD CNP BNP GCase GC-B GC-A extracellular domain fluorescence-activated cell sorting C-type natriuretic peptide brain natriuretic peptide atrial natriuretic peptide guanylyl cyclase