Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10761443 | Biochemical and Biophysical Research Communications | 2012 | 6 Pages |
Abstract
⺠Engineered kinesin-M13 and calmodulin involving single cysteine were prepared. ⺠CaM mutant was cross-linked to dimer by bifunctional thiol reactive reagent. ⺠Kinesin-M13 was dimerized via CaM dimer in the presence of calcium. ⺠Function of the engineered kinesin was regulated by a Ca2+-calmodulin dimer linker.
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Authors
Hideki Shishido, Shinsaku Maruta,