Copy number of adenoviral vector genome transduced into target cells can be measured using quantitative PCR: Application to vector titration

Article ID	Journal	Published Year	Pages	File Type
10761713	Biochemical and Biophysical Research Communications	2012	6 Pages	PDF

Abstract

âº The copy number of internalized adenoviral vector DNA can be measured using qPCR. âº Viral DNA and inactivated viral particles on the cell surface can be washed out. âº Titration method of the adenoviral vector using quantitative PCR was established. âº The viral titer shows the transduced copy number of viral genome in target cells. âº Current titration methods may be replaced by this new titration method using qPCR.

Keywords

qPCR RVT TCID50 NLS GIT OTC CPE AdV MOI Quantitative PCR Cytopathic effect Adenoviral vector Adenovirus vector Ornithine transcarbamylase Virus titer nuclear localization signal plaque-forming unit quantitative real-time PCR pfu multiplicity of infection