PI3K/Akt contributes to increased expression of Toll-like receptor 4 in macrophages exposed to hypoxic stress

Article ID	Journal	Published Year	Pages	File Type
10761982	Biochemical and Biophysical Research Communications	2012	6 Pages	PDF

Abstract

âº Hypoxic stress-induced TLR4 expression is mediated by PI3K/Akt in macrophages. âº PI3K/Akt regulated HIF-1 activation leading to TLR4 expression. âº p38 mitogen-activated protein kinase was not involved in TLR4 expression by hypoxic stress. âº Sulforaphane suppressed hypoxia-mediated TLR4 expression by inhibiting PI3K/Akt.

Keywords

PI3K LPS RLA CoCl2 MFI DIC MYD88 HIF-1 MAL TLR GSK-3 TIRAP SFN 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate DiI TRIF Akt Hypoxic stress TRAM Toll-like receptor Sulforaphane hypoxia-inducible factor-1 phosphoinositide 3-kinase relative luciferase activity lipopolysaccharide TRIF-related adaptor molecule mean fluorescence intensity veh Vehicle myeloid differentiation primary response gene 88 Cobalt chloride differential interference contrast glycogen synthase kinase 3