| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10763108 | Biochemical and Biophysical Research Communications | 2011 | 6 Pages |
Abstract
⺠We transfect preadipocytes stably with antisense lipocalin-type PGD synthase. ⺠The transfection specifically suppresses the synthesis of prostaglandins D2. ⺠The cloned transfectants exhibit a significant stimulation of fat storage. ⺠Up-regulation of adipogenesis occurs in a PG-independent manner in those cells.
Keywords
COXlipocalin-type PGD synthaseLPL15d-PGJ2G418AP2PPARγRT-PCRFBSL-PGDSAdipocytecyclooxygenaseStable transfectionELISAEnzyme-linked immunosorbent assayfetal bovine serumLipoprotein lipaselipocalin-type prostaglandin D synthaseCyclooxygenase pathwayReverse transcriptase-polymerase chain reactionadipocyte protein 2prostaglandinGeneticinGlut-4glucose transporter-4peroxisome proliferator-activated receptor γ
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Authors
Abu Asad Chowdhury, Mohammad Salim Hossain, Mohammad Sharifur Rahman, Kohji Nishimura, Mitsuo Jisaka, Tsutomu Nagaya, Fumiaki Shono, Kazushige Yokota,