Anti-proliferative actions of 2-decylamino-5,8-dimethoxy-1,4-naphthoquinone in vascular smooth muscle cells

Article ID	Journal	Published Year	Pages	File Type
10763415	Biochemical and Biophysical Research Communications	2011	6 Pages	PDF

Abstract

âº 2-Decylamino-DMNQ inhibited PDGF-BB-induced VSMC proliferation in a dose-dependent manner with no apparent cytotoxicity. âº 2-Decylamino-DMNQ inhibited PDGF-BB-induced phosphorylation of Erk1/2 and PLCÎ³1. âº 2-Decylamino-DMNQ arrested a G0/G1 cell cycle progression in association with pRb phosphorylation and PCNA expression. âº Both U0126, an Erk inhibitor, and U73122, a PLCÎ³ inhibitor, arrested a G0/G1 phase of the cell cycle.

Keywords

PRB PDGF-Rβ PCNA VSMCs PDGF receptor β PDGF PLCγ CDK PBS DMEM FBS DMSO ERK1/2 MAPK SDS–PAGE proliferative cell nuclear antigen sodium dodecyl sulfate–polyacrylamide gel electrophoresis restenosis Dimethylsulfoxide fetal bovine serum Vascular smooth muscle cell Vascular smooth muscle cells platelet-derived growth factor Phosphate buffered saline Dulbecco’s modified eagle’s medium naphthoquinone retinoblastoma protein mitogen-activated protein kinase Propidium iodide Cell cycle cyclin-dependent kinase