| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10764079 | Biochemical and Biophysical Research Communications | 2011 | 5 Pages |
Abstract
⺠Posttranscriptional regulation of miRNA processing is difficult to quantify. ⺠Our in-vivo processing assay can quantify Drosha cleavage in live cells. ⺠It is based on luciferase reporters fused with pri-miRNAs. ⺠The assay validates the processing defect caused by a mutation in pri-16-1. ⺠It is a sensitive method to quantify pri-miRNA cleavage by Drosha in live cells.
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Authors
Danilo Allegra, Daniel Mertens,