Evidence for involvement of the C-terminal domain in the dimerization of the CopY repressor protein from Enterococcus hirae

► A metal-binding protein domain is directly involved in protein dimerization. ► Fusing the metal-binding domain to a monomeric protein induces dimerization. ► Frontal size-exclusion chromatography measures the strength of dimer interaction. ► Ultracentrifugation studies confirm the influence of metal binding on dimerization.