Extrusion of the C-terminal helix in navel orangeworm moth pheromone-binding protein (AtraPBP1) controls pheromone binding

Article ID	Journal	Published Year	Pages	File Type
10764524	Biochemical and Biophysical Research Communications	2011	4 Pages	PDF

Abstract

âº pH-dependent pheromone binding to moth pheromone-binding protein (AtraPBP1). âº C-terminal helix blocks pheromone binding at low pH. âº C-terminal deletion promotes pheromone binding at low pH. âº H80A and H95A mutants promote pheromone binding at low pH. âº Extrusion of C-terminal helix controls pH-dependent pheromone binding.

Keywords

SEC IPTG HSQC PBP Amyelois transitella SDS–PAGE sodium dodecylsulfate–polyacrylamide gel electrophoresis NMR Pheromone Pheromone-binding protein Disulfide bridge Size exclusion chromatography heteronuclear single quantum coherence