| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10764569 | Biochemical and Biophysical Research Communications | 2011 | 4 Pages |
Abstract
⺠A new method of monitoring ER Ca2+ directly in live cells. ⺠Confocal microscopy and low affinity Ca2+ indicator, Fluo5F, reveals ER Ca2+ morphology. ⺠Fluo5F fluorescence shows no co-localization with mitochondria or cytosol. ⺠Stimulation of ER Ca2+ release reveal spatiotemporal differences in ER Ca2+ release dynamics.
Keywords
IP3RPTCtetramethyl rhodamine methyl esterLSCMTMRMinositol triphosphateFCCPEGTADMSOethylene glycol tetraacetic aciddiacylglycerolDimethyl sulfoxideDAGRenal proximal tubular cellendoplasmic reticulumSERCAConfocal microscopyLaser scanning confocal microscopycarbonylcyanide-p-trifluoromethoxyphenylhydrazoneCalciumKidney
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Authors
Andre C. Eaddy, Rick G. Schnellmann,