Article ID Journal Published Year Pages File Type
10764763 Biochemical and Biophysical Research Communications 2010 5 Pages PDF
Abstract
► NBD-ceramide-based assays to monitor ceramide metabolism and compare ceramide kinase, glucosylceramide synthase and sphingomyelin synthase activities. ► Analysis of ceramide metabolism enzyme activities during macrophage homeostasis (elicitated/resident, macrophage/monocyte, innate activation, classical activation, alternative activation, de-activation by glucocorticoids). ► Identification of “ceramide anabolic switching”: ceramide kinase and glucosylceramide synthase appear to be regulated in opposite directions.
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