Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10765035 | Biochemical and Biophysical Research Communications | 2010 | 5 Pages |
Abstract
Naofen has recently been identified from the rat brain/spinal cord cDNA library as a substance reactive against an anti-shigatoxin (Stx)-2 antibody. Naofen mRNA is composed of 4620 nucleotides and encodes 1170 amino acids. Naofen contains four WD-repeat domains in its N-terminus and is ubiquitously distributed in many tissues of the rat. Tumor necrosis factor (TNF)-α enhanced the expression of naofen mRNA in HEK293 cells in a dose-dependent manner. Furthermore, naofen siRNA, which predominantly knocked down the expression of naofen mRNA, significantly reduced both TNF-α-induced caspase-3 activation and apoptosis in HEK293 cells. Overexpression of naofen in HEK293 cells (FLAG-NF) spontaneously induced caspase -3 activation and apoptosis, and showed extremely high susceptibility to TNF-α-induced apoptosis. These results indicated that naofen may function as a novel modulator activating caspase-3, and promoting TNF-α-stimulated apoptosis.
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Authors
Guo-Gang Feng, Chang Li, Lei Huang, Koji Tsunekawa, Yuko Sato, Yoshihiro Fujiwara, Tooru Komatsu, Takashi Honda, Jun-Hua Fan, Hidemi Goto, Tatsuro Koide, Takaaki Hasegawa, Naohisa Ishikawa,