| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10765324 | Biochemical and Biophysical Research Communications | 2010 | 5 Pages |
Abstract
HDAC inhibitors induce cell cycle arrest of E1A + Ras-transformed cells accompanied by e2f1 gene down-regulation and activation of Wnt pathway. Here we show that e2f1 expression is regulated through the Wnt/Tcf-pathway: e2f1 promoter activity is inhibited by sodium butyrate (NaB) and by overexpression of β-catenin/Tcf. The e2f1 promoter was found to contain two putative Tcf-binding elements: the proximal one competes well with canonical Tcf element in DNA-binding assay. Being inserted into luciferase reporter vector, the identified element provides positive transcriptional regulation in response to β-catenin/Tcf co-transfection and NaB treatment. Thus we have firstly demonstrated that e2f1 belongs to genes regulated through Wnt/β-catenin/Tcf pathway.
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Authors
Maria V. Abramova, Evgeny A. Zatulovskiy, Svetlana B. Svetlikova, Alexander N. Kukushkin, Valery A. Pospelov,