Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10766629 | Biochemical and Biophysical Research Communications | 2008 | 5 Pages |
Abstract
Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-β-d-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves.
Keywords
TOFHMBCDianthus caryophyllusBAHDAnthocyanin acyltransferaseAATCOAAcyltransferaseTHFESInuclear magnetic resonanceAnthocyanin2-Morpholinoethanesulfonic acidMalic acidTetrahydrofuranNMRtime of flightCyanidin 3-glucosideMass spectrometryMeSheteronuclear multiple bond connectivityCarnationHPLChigh-performance liquid chromatographycoenzyme Aelectrospray ionization
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Authors
Yutaka Abe, Masayuki Tera, Nobuhiro Sasaki, Masachika Okamura, Naoyuki Umemoto, Masaki Momose, Nobuo Kawahara, Hiroyuki Kamakura, Yukihiro Goda, Kazuo Nagasawa, Yoshihiro Ozeki,