Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10766660 | Biochemical and Biophysical Research Communications | 2008 | 6 Pages |
Abstract
As the transcriptional activity of hypoxia-inducible factor 1 (HIF-1) is associated with resistance of tumor cells to current antitumor therapies, the spatiotemporal dynamics of HIF-1-active regions has been of great interest as a therapeutic target. In the present study, we established a unique cancer cell line, which changes color HIF-1-dependently, and monitored it during tumor progression. In imaging experiments, HIF-1-active cells appeared over the tumor xenograft, but dramatically decreased in number as blood vessels developed around the tumor. The remaining HIF-1-active cells at the center of the xenograft also disappeared after neovascularization. Thereafter, tumor growth was accelerated and HIF-1-active cells reappeared in different regions. The distance between HIF-1-active cells and the nearest vessels correlated to the diameter of the vessel (r = 0.801). These results provide a basic knowledge of how to estimate the spatiotemporal dynamics of HIF-1-active cells using information about the image-guided architecture of tumor blood vessels.
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Authors
Hiroshi Harada, Xuejun Xie, Satoshi Itasaka, Lihua Zeng, Yuxi Zhu, Akiyo Morinibu, Kazumi Shinomiya, Masahiro Hiraoka,