Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10766729 | Biochemical and Biophysical Research Communications | 2008 | 7 Pages |
Abstract
TR2 (TNFR-related 2, HVEM, or TNFRSF-14), a member of the TNFR family, is involved in a number of immune responses. While TR2 is expressed on the surface of T cells during the resting state, little is known regarding how expression of the TR2 gene is regulated. To understand the mechanisms regulating the expression of TR2 in T cells, we analyzed the 5' flanking region of TR2. We identified an important region for the activity of the TR2 promoter using site directed mutagenesis. Using EMSA analysis, we found that IRF-2 was bound to the promoter region of the TR2 gene during the resting state of EL-4 T cells. Transfection of IRF-2 expression plasmid and of dominant negative IRF-2 mutant further confirmed our results. Together, these data demonstrate that IRF-2 is involved in the regulation of TR2 expression in EL-4 T cells.
Keywords
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Biochemistry
Authors
Ok-Ju Sul, Hyeon-Woo Lee, Woon-Ki Kim, Jin-Hee Choi, Jung-Sook Kwak, Youn-Jung Choi, Anne M. Latour, Beverly H. Koller, Choon-Soo Jeong, Byoung S. Kwon,