Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10766932 | Biochemical and Biophysical Research Communications | 2007 | 7 Pages |
Abstract
Guggulsterone (GS) and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] have been shown to influence adipogenesis in 3T3-L1 cells. We investigated the ability of GS and 1,25(OH)2D3, alone and in combination to inhibit adipogenesis and induce apoptosis in 3T3-L1 adipocytes. Maturing preadipocytes were treated with 1,25(OH)2D3 in combination with GS for 6Â days during differentiation. GS and 1,25(OH)2D3 each inhibited lipid accumulation, but the combination potentiated the inhibition of lipid accumulation. Apoptosis was increased by 1,25(OH)2D3 while GS had no effect, but GSÂ +Â 1,25(OH)2D3 increased apoptosis more than either compound alone. Furthermore, GSÂ +Â 1,25(OH)2D3 caused a potentiated decrease in the expression of aP2 and farnesoid X receptor expression more than either compound alone. In addition, 1,25(OH)2D3 increased vitamin D receptor expression after 6Â days, while GS had no effect. GSÂ +Â 1,25(OH)2D3, however, caused a potentiated increase in the expression of VDR. These findings show that GS potentiates 1,25(OH)2D3's anti-adipogenic and pro-apoptotic effects in maturing 3T3-L1 preadipocytes.
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Authors
Srujana Rayalam, Mary Anne Della-Fera, Suresh Ambati, Barbara Boyan, Clifton A. Baile,