Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10767290 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
The KAI1/CD82 protein has been documented as the tumor metastasis suppressor in many types of human cancers. KAI1/CD82 regulates cell motility and invasiveness; however, the mechanism by which this occurs remains to be fully established. Several studies have shown that KAI1/CD82 modulates integrin-dependent signaling. It was suggested that KAI1/CD82 might function to attenuate the β1 integrin function of inducing cellular migration. A wound-healing and modified Boyden chamber assays were performed to investigate the mechanism of the KAI1/CD82-mediated inhibition of cell migration. It was found that the migratory ability of H1299/CD82 was inhibited. The immunoblotting and biotinylation assays revealed that H1299/CD82 showed significantly decreased expression of the mature form of β1, which was functional at the cell surface. It was confirmed that KAI1/CD82 regulates the maturation of the β1 integrin using CD82-specific si-RNA. These results support a model in which KAI1/CD82 attenuates the maturation of the β1 integrin precursor and thereby suppresses cell migration.
Keywords
ECMKAI1/CD82H1299Duffy Antigen Receptor for ChemokinesTGF-βSfMFAKTM4SFmAbPBSBSAHGF/SFbovine serum albuminMonoclonal antibodyIntegrin β1transforming growth factor-βDARCSerum free mediaTumor suppressorendoplasmic reticulumHepatocyte growth factor/scatter factorTransmembrane 4 superfamilyFibronectinLamininExtracellular matrixPhosphate-buffered salineCell migrationfocal adhesion kinase
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Authors
Bo Keun Jee, Joo Yong Lee, Young Lim, Kweon Haeng Lee, Yang-Hyeok Jo,