Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10767329 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
Human prostate and colon gene-1 (PC-1, also known as PrLZ) is an androgen-regulated, prostate tissue and prostate cancer cells specifically expressed novel gene. The increased expression of PC-1 gene appears to promote prostate cancer cells androgen-dependent (AD) and androgen-independent (AI) growth. To clone and investigate the expression and regulation elements of PC-1 gene may provide insight into the function of PC-1 and develop a new promoter that targets therapeutic genes to the AD and AI prostate cancer cells. The goal of the present study is cloning and characterization of the PC-1 promoter. A series of luciferase constructs that contain various fragments of the PC-1 5â²-genomic region were transfected into human prostate cancer cells for promoter transactivation analysis. 5â² deletion analysis identified the â1579Â bp promoter region was required for the maximal proximal promoter activity; two transcriptional suppression and a positive regulatory region were identified; â4939Â bp promoter fragment of the PC-1 gene retained the characteristic of prostate cancer-specific expression and exhibited higher transcription activity than PSA-6Â kb promoter in the medium supplemented with steroid-depleted FBS. An androgen response element (ARE) was located in between â345 and â359Â bp of the PC-1 5â²-untranslated region relative to the translation initiation site. Thus, our studies not only provide molecular basis of PC-1 transcription regulation, but also define a new regulatory sequence that may be used to restrict expression of therapeutic genes to prostate cancer in the prostate cancer gene therapy.
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Authors
Jian Wang, Hui Zhang, Rui-Xia Liang, Bo Pang, Qing-Guo Shi, Pei-Tang Huang, Cui-Fen Huang, Jian-Guang Zhou,