| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10767339 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
We have investigated the efficiency of N-terminal 1-260 residues of Edema factor (EFn) as a delivery system for ESAT-6, an antigenic protein of Mycobacterium tuberculosis H37Rv, into the cytosol of mammalian cells. The EFn.ESAT-6 recombinant protein was obtained by genetic fusion of EFn and ESAT-6 DNA. Our data shows that in the presence of PA, EFn.ESAT-6 fusion protein is internalized into the cytosol of antigen presenting cells, and the splenocytes produced both Th1 and Th2 cytokines in vitro. Further, EFn.ESAT-6 elicited effective cytotoxicT lymphocyte (CTL) response in an in vitro CTL assay. This study for the first time demonstrates that EFn can be used as a vehicle to deliver heterologous proteins of therapeutic importance.
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Authors
Subhash Chandra, Manpreet Kaur, Shuchi Midha, Jyotsna Gorantala, Rakesh Bhatnagar,