Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10767419 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
It is widely accepted that the vesicle-transporter myosin-V moves processively along F-actin with large steps of â¼36Â nm using a hand-over-hand mechanism. A key question is how does the rear head of two-headed myosin-V search for the forward actin target in the forward direction. Scanning probe nanometry was used to resolve this underlying search process, which was made possible by attaching the head to a relatively large probe. One-headed myosin-V undergoes directional diffusion with â¼5.5Â nm substeps to develop an average displacement of â¼20Â nm, which was independent of the neck length (2IQ and 6IQ motifs). Two-headed myosin-V showed several â¼5.5Â nm substeps within each processive â¼36Â nm step. These results suggest that the myosin-V head searches in the forward direction for the actin target using directional diffusion on the actin subunits according to a potential slope created along the actin helix.
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Authors
Takuya Okada, Hiroto Tanaka, Atsuko Hikikoshi Iwane, Kazuo Kitamura, Mitsuo Ikebe, Toshio Yanagida,