Article ID Journal Published Year Pages File Type
10767583 Biochemical and Biophysical Research Communications 2007 7 Pages PDF
Abstract
Hypoxia-inducible factors (HIFs) are key mediators of cellular adaptation to hypoxia, but also respond to non-hypoxic stimuli. To clarify involvement in metabolic disturbances, HIFs were characterised in rats subjected to insulin-induced hypoglycaemia or cellular glucoprivation provoked by 2-deoxy-d-glucose (2-DG). Using real-time qPCR, organ-specific expression of HIF-1α, -2α, -3α, -1β, and of the target gene GLUT-1 was determined. Distribution of HIF-3α proteins was examined by immunohistochemistry. Both, insulin and 2-DG resulted in a widespread increase in HIF-3α mRNA. HIF-2α mRNA increased in lung and heart after 2-DG only, whereas other HIFs remained unaffected. A pronounced increase of protein levels in cerebral cortex was observed for HIF-3α. Functional significance of HIF induction was reflected in enhancement of GLUT-1 mRNA. Transcriptional up-regulation of HIF-3α represents a typical response to in vivo hypoglycaemia and glucoprivation. These data suggest an involvement of the HIF system in metabolic derangements as for instance caused by diabetes.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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