Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10767584 | Biochemical and Biophysical Research Communications | 2007 | 5 Pages |
Abstract
Physical interaction between the cell surface receptors CD47 and signal regulatory protein alpha (SIRPα) was reported to regulate cell migration, phagocytosis, cytokine production, and macrophage fusion. However, it is unclear if the CD47/SIRPα-interaction can also regulate macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB ligand (RANKL)-stimulated formation of osteoclasts. Here, we show that functional blocking antibodies to either CD47 or SIRPα strongly reduced formation of multinucleated tartrate-resistant acid phosphatase (TRAP)+ osteoclasts in cultures of murine hematopoietic cells, stimulated in vitro by M-CSF and RANKL. In addition, the numbers of osteoclasts formed in M-CSF/RANKL-stimulated bone marrow macrophage cultures from CD47â/â mice were strongly reduced, and bones of CD47â/â mice exhibited significantly reduced osteoclast numbers, as compared with wild-type controls. We conclude that the CD47/SIRPα interaction is important for M-CSF/RANKL-stimulated osteoclast formation both in vivo and in vitro, and that absence of CD47 results in decreased numbers of osteoclasts in CD47â/â mice.
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Authors
Pernilla Lundberg, Cecilia Koskinen, Paul A. Baldock, Hanna Löthgren, Ã
sa Stenberg, Ulf H. Lerner, Per-Arne Oldenborg,