Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10767837 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
In this study, we determined the efficacy of minocycline, a second generation tetracycline, in preventing male germ cell apoptosis after withdrawal of gonadotropins and intratesticular testosterone (T). Groups of 5 male rats received one of the following treatments daily for 5 days: (i) daily sc injection of GnRH-A (1.6Â mg/kg BW), (ii) oral administration of 30% gum acacia as a vehicle control, and (iii) GnRH-AÂ +Â oral administration of 50 or 100Â mg/kg BW of minocycline. Minocycline at both 50 and 100Â mg dose levels significantly (PÂ <Â 0.05) prevented GnRH-A -induced germ cell apoptosis by 59.4% and 62.2%, respectively, and fully prevented PARP cleavage. Minocycline-mediated protection occurred at the mitochondria, involving the restoration of the BCL-2 levels and, in turn, suppression of cytochrome c and DIABLO release. Minocycline was also effective in preventing human male germ cell apoptosis induced by hormone free culture condition.
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Authors
Mark Castanares, Yanira Vera, Krista Erkkilä, Sauli Kyttänen, Yanhe Lue, Leo Dunkel, Christina Wang, Ronald S. Swerdloff, Amiya P. Sinha Hikim,