Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10768249 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
To investigate the maturation of neonatal erythrocytes and kinetics of globin expression, we induced CD34+ cells purified from human umbilical cord blood to erythroid differentiation in different suspension culture systems containing human cord serum (CHS) and adult serum (AHS), respectively. The ζ- to α-globin switching and the ε- to γ-globin switching were observed in CHS+ cultures but not in AHS+ cultures. A reduced proportion of F cell and two day postponed cell enucleation in AHS+ cultures compared with in CHS+ cultures was also found. However, predominant γ-globin expression compared with ε- and β-globin was always observed from days 5 in either CHS+ or AHS+ cultures. Our data showed that neonatal erythrocytes in CHS+ cultures maintained their fetal characteristics which differed from their counterpart from adult. Serum from different developmental stage significantly affected the maturation and globin expression pattern of cultured neonatal erythrocytes. Obviously, some uncertain serum components play important roles in cultures, but they are not enough to fundamentally change the developmentally specific globin gene transcription program, suggesting that this program is mainly controlled by determinants within the erythroid progenitors.
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Authors
Xin Zhang, Yuan-Ming Luo, Yan-Ni Ma, Jun-Wu Zhang,