Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10768469 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total â¼27) and ModB (total â¼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.
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Authors
Reinhard Depping, Christiane Lohaus, Helmut E. Meyer, Wolfgang Rüger,