| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10768488 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
A rat model for human minimal change nephropathy was obtained by the intravenous injection of adriamycin (ADR) at 5Â mg/kg. By using an in vivo electron paramagnetic resonance (EPR) spectrometer operating at 700Â MHz, the temporal changes in signal intensities of a nitroxide radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), in the kidneys of rats with ADR nephropathy were investigated. The decay rate of the EPR signal intensity obtained in the kidney is indicative of the renal reducing ability. It was found that the reducing ability in the kidney declined on the 7th day after ADR administration and recovered after the 14th day. Impairment of the reducing ability occurred before the appearance of continuous urinary protein. The in vitro EPR study showed that this impairment of in vivo renal reducing ability is related to impairment of the reducing ability in the mitochondria.
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Authors
Takaaki Oteki, Sohji Nagase, Hidekatsu Yokoyama, Hiroaki Ohya, Takao Akatsuka, Mika Tada, Atsushi Ueda, Aki Hirayama, Akio koyama,