Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10768553 | Biochemical and Biophysical Research Communications | 2005 | 9 Pages |
Abstract
Chondrocyte glycosaminoglycan (GAG) synthesis is regulated by the availability of UDP-glucuronate, the substrate of glucuronosyl transferases which form the GAG chains in proteoglycans and hyaluronan. UDP-glucose dehydrogenase (UDPGD) is therefore a key enzyme in the synthesis of UDP-glucuronate from glucose. However, the mechanisms regulating its expression in chondrocytes are not fully understood. We investigated the effect of c-Krox, a zinc-finger transcription factor previously shown to modulate several matrix genes, on the synthesis of GAG and transcriptional activity of several UDPGD gene promoter constructs, using transient transfection and decoy experiments in rabbit articular chondrocytes (RACs). We show that overexpression of c-Krox inhibits radiosulfate incorporation into neosynthesized GAG and that the effect was mediated by a cis-sequence located between +18 and +39Â bp of the UDPGD gene. Since that sequence can also bind Sp1/Sp3 factors, it is likely that c-Krox acts in concert with these proteins to modulate the UDPGD gene expression in articular chondrocytes.
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Authors
Gallic Beauchef, Magdalini Kypriotou, Christos Chadjichristos, Russell L. Widom, Benoît Porée, Emmanuelle Renard, Safa Moslemi, Yanusz Wegrowski, François-Xavier Maquart, Jean-Pierre Pujol, Philippe Galéra,