Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10768919 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
This study was undertaken to reveal the role of NAD(P)H oxidase in increased oxidative stress in islets of Type 2 diabetes. Immunostaining analysis showed that staining intensities of NAD(P)H oxidase components, gp91phox and p22phox, significantly increased in islets of animal models of Type 2 diabetes, OLETF rats (60 weeks of age) and db/db mice (14 weeks of age), compared with age-matched controls, respectively, correlating with increased levels of oxidative stress marker, 8-hydroxy-deoxyguanosine or 4-hydroxy-2-nonenal modified protein. In db/db mice, oral administration of angiotensin II Type 1 receptor antagonist valsartan (5 mg/kg) for 4 weeks significantly attenuated the increased expression of gp91phox and p22phox together with inhibition of oxidative stress and partially restored decreased insulin contents in islets. Angiotensin II-related increased expression of NAD(P)H oxidase may play an important role in increased oxidative stress in islets of Type 2 diabetes. This mechanism may be a novel therapeutic target for preventing β-cell damage.
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Authors
Mieko Nakayama, Toyoshi Inoguchi, Toshiyo Sonta, Yasutaka Maeda, Shuji Sasaki, Fumi Sawada, Hirotaka Tsubouchi, Noriyuki Sonoda, Kunihisa Kobayashi, Hideki Sumimoto, Hajime Nawata,